Yet, only a few beetle genomes have been sequenced so far [2,3], and the genomic resources available to study molecular evolution in beetles are very limited relative to many other groups. https://doi.org/10.1371/journal.pone.0158565.t001. if(MSFPhover) { MSFPnav14n=MSFPpreload("../Portrait-Seiten/_derived/Kaefer-Portrait-1.htm_cmp_Ripple110_vbtn.gif"); MSFPnav14h=MSFPpreload("../Portrait-Seiten/_derived/Kaefer-Portrait-1.htm_cmp_Ripple110_vbtn_a.gif"); } In an attempt to better understand the origin of our duplicates, we ran the same BUSCO analysis using the three individual assemblies. if(MSFPhover) { MSFPnav23n=MSFPpreload("../Portrait-Seiten/_derived/Zweifluegler-Portrait-3.htm_cmp_Ripple110_vbtn.gif"); MSFPnav23h=MSFPpreload("../Portrait-Seiten/_derived/Zweifluegler-Portrait-3.htm_cmp_Ripple110_vbtn_a.gif"); } Die This step reduces the number of reads, but increases the quality of the assembly by removing non-uniform and redundant k-mer reads, thus improving the assembly process time. if(MSFPhover) { MSFPnav31n=MSFPpreload("../Portrait-Seiten/_derived/Pilze-Portrait.htm_cmp_Ripple110_vbtn.gif"); MSFPnav31h=MSFPpreload("../Portrait-Seiten/_derived/Pilze-Portrait.htm_cmp_Ripple110_vbtn_a.gif"); } Males tend to be smaller and possess a more rotund shape than the female. Tektin is an essential protein for the development of cilia and flagella. In total, 33,216 putative genes were successfully annotated using Blastx against the Nr (non-redundant) database and 13,382 were assigned to 34,100 Gene Ontology (GO) terms. if(MSFPhover) { MSFPnav15n=MSFPpreload("../Portrait-Seiten/_derived/Kaefer-Portrait-2.htm_cmp_Ripple110_vbtn.gif"); MSFPnav15h=MSFPpreload("../Portrait-Seiten/_derived/Kaefer-Portrait-2.htm_cmp_Ripple110_vbtn_a.gif"); } The overall workflow, summarizing the transcriptome assembly steps, is presented graphically in Fig 1. https://doi.org/10.1371/journal.pone.0158565.g001. In total, 492 million paired-end reads were assembled into a high number of genes (145,883), of which 33,216 were annotated. Seed beetles have a very rapid life cycle (about three weeks). // -->